In this study, the antioxidant activities of different parts of lotus (Nelumbo nuficera Gaertn) rhizome were compared. The total phenolic content of lotus rhizome was determined, and Ferric reducing antioxidant power (FRAP) assay, 1,1-diphenyl-2-picrylhydrazyl hydrate (DPPH) radical-scavenging assay and β-carotene-linoleic acid assay were performed to assess the antioxidant activity of lotus rhizome. Results showed that there was a significant difference in total phenolic content and antioxidant activity between any two of four parts of lotus rhizome. The order of total phenolic content and antioxidant activity in different parts of lotus rhizome was as follows: peel of old lotus rhizome > peel of young lotus rhizome > flesh of old lotus rhizome > flesh of young lotus rhizome. The total phenol content is significantly positive correlated with the antioxidant activity in different parts of lotus rhizome. This study has provided a basis for further exploring the antioxidant components in lotus rhizome.