N2fixation driven by mechanical energy is a promising strategy for production of nitrogen-enriched compounds. However, the activity of mechanical-energy-driven N2fixation is very low. Herein, a mechanical-energy-driven triboelectric plasma jet was constructed to achieve N2fixation in air at room temperature and atmospheric pressure. Under optimal conditions, the NOXproduction rate of 4.82 μmol h−1is 23-fold better than the previous record using a triboelectric nanogenerator. The electrical to chemical energy conversion efficiency and energy cost for NOXproduction are 4.92% and 1.76 MJ mol−1N−1, respectively, and the energy cost is the best result in the reported plasma N2fixation reaction at room temperature and atmospheric pressure. Because of the lower average energy of electrons in the triboelectric plasma jet, the vibrational excitation dissociation process with low energy barriers is the major mechanism for N2fixation. This study provides an effective strategy for N2fixation using mechanical energy.