The microstructure and mechanical properties of an X100 grade pipeline steel were investigated by the thermal simulation tests performed on a Gleeble-3500 thermal simulator. The results show that the steel has excellent weldability. Even if the weld heat input gets 40 kJ mm-1, the low impact toughness energy in welding coarse grain heat affected zone (CGHAZ) is still higher than 200 J. Yet, an embrittlement zone emerges in inter-critical HAZ (ICHAZ) at the peak temperature of 750 °C, and the lowest strength appears in fine grained HAZ (FGHAZ) at the peak temperature of 950 °C. Moreover, the strength and toughness decrease with the increase of heat input.