Europium‐Labeled Activity‐Based Probe through Click Chemistry: Absolute Serine Protease Quantification Using 153Eu Isotope Dilution ICP/MS
- Resource Type
- Article
- Authors
- Yan, Xiaowen; Luo, Yacui; Zhang, Zhubao; Li, Zhaoxin; Luo, Qiang; Yang, Limin; Zhang, Bo; Chen, Haifeng; Bai, Peiming; Wang, Qiuquan
- Source
- Angewandte Chemie. International Edition; April 2012, Vol. 51 Issue: 14 p3358-3363, 6p
- Subject
- Language
- ISSN
- 14337851; 15213773
Click and analyze: The titled probe was synthesized by conjugating a sulfonyl fluoride and azido unit using click chemistry to give SF‐Eu, which can react specifically with serine (Ser) in the active site of serine protease (SP). Combination of the method with 153Eu‐isotope dilution ICP/MS enables absolute protein quantification of active SPs in biological samples using only one 153Eu(NO3)3isotopic standard.