The Mn oxides generated by microorganisms are of great interest recently, due to high reactivity and adsorption capacity. The biological Mn oxidation by Pseudomonas putida strain MnB1 (P. putida MnB1) and heavy metal adsorption by the generated biogenic Mn oxides (BMO) were investigated. The nano-sized and poorly crystalline BMO was generated by P. putida MnB1. The Mn(II) oxidation was inhibited in the presence of heavy metals such as Fe(III) or Pb(II). The adsorption capacities of Pb(II), Cd(II), and Zn(II) were 7-8 times higher than those of birnessite. Pb(II) was adsorbed preferentially to Cd(II) or Zn(II) probably because of the higher electronegativity and lower hydration energy. Heavy metals adsorption capacity increased as pH increased, as temperature increased, and as ionic strength decreased. The results in this study indicate that BMO is an excellent adsorbent due to the abundant vacancies in the amorphous structure which can incorporate heavy metals. However, since the BMO generated by P. putida MnB1 is nano-sized, it is not easy to separate BMO from the water after heavy metal adsorbed by it. In this way, immobilization was introduced in this study. Silicate, alumina, zeolite, CNT, EG, AC, sand (quartz) could be the support of BMO. Among them, zeolite was chosen to be the support due to its mesoporous property, highly surface area and highly adsorption capacity. The adsorption kinetic of immobilization was studied. Heavy metal adsorption by BMO immobilized zeolite (Mn/Z) and natural zeolite (N/Z), NaOH treated zeolite (OH/Z) and NaCl treated zeolite (Cl/Z) were also studied. It was found that the heavy metal adsorption capacity of Cl/Z was greater than OH/Z and N/Z, but was less than Mn/Z. The heavy metal adsorption order is: Mn/Z > Cl/Z > OH/Z > N/Z. The effect factors including pH, temperature and ionic strength were observed in this study. Heavy metal adsorption capacity increased as pH increased, as temperature increased, and as ionic strength decreased. Single-heavy metal and multi-heavy metals equilibrium data were measured experimentally to observe the competition effect of heavy metals. Pb was preferentially than Cd and Zn in the multi-heavy metal system which indicant that Pb has far more greater affinity to BMO and Mn/Z.The BMO and Mn/Z are therefore a promising tool and a novel adsorbent not only for the adsorption of heavy metal, but also for the removal of organic compounds. In the further study, the removal of the organic compounds such as 17α-ethinyletradiol (EE2) by the novel adsorbent was investigated in this study, which will provide some new insights into the interaction of contaminated water treatment.