Phosphorylation, a ubiquitous regulatory mechanism of diverse protein functions, exerts critical roles in regulating various cellular processes. In order to obtain easy and rapid protocol to visualize phosphoprotein in SDS-PAGE, five fluorescent detection methods named Alizarin red S, Morin hydrate, Calcon, Fura 2 pentapotassium salt and 8-Quinolinol stains were developed. Al3+ was applied as a “fixed bridge”, providing an efficient energy transfer channel between phosphoprotein and dye, to produce a strong fluorescent complex for the determination of phosphoprotein. The system employed the Alizarin red S-aluminum (III)-appended complex as a fluorescent staining dye to perform the convenient and selective detection of phosphorylated proteins and total proteins in SDS-PAGE, respectively. Therefore, a full and selective map of proteins can be achieved in the same process without resorting to other compatible detection methods. As low as 62.5 ng of α-casein (7 or 8 phosphates) and β-casein (5 phosphates), 125 ng of ovalbumin (2 phosphates) and κ-casein (1 phosphate) can be detected in approximately 135 min, with the linear responses of rigorous quantitation of changes over a 125-4000 ng range.For Morin hydrate, as little as 62.5 ng of α-casein and β-casein, 125 ng of ovalbumin and κ-casein could be visualized with a wide linear dynamic range. In comparison with conventional methods, Morin hydrate stain is a time saving method which takes just 90 min. It also has good compatibility with routine protein stainings such as Coomassie Brilliant Blue R and SYPRO Ruby for total protein analysis. Calcon stain delivers results in 135 min, and the sensitivities are 31 ng of α-casein and β-casein, 62.5 ng of κ-casein and 125 ng of ovalbumin, respectively. Furthermore, the staining yields negative signals for some non-phosphoproteins that are distinctly different from that of positive signals of phosphoproteins. Therefore, this staining method allows phosphoproteins to be monitored with high specificity. Additionally, compared with traditional techniques, it offers several advantages, including simple operation with a good linear dynamic range, no requirement for expensive chemicals, and achievement of a total proteins profile by a scanner before destaining. As a fluorescent dye and a metal ion, Fura 2 pentapotassium salt and Al3+ were employed, respectively, to detect phosphoproteins. The staining method, Fura 2 pentapotassium salt stain, has sensitivities of 16-31 ng of α-casein and β-casein, 62.5 ng of ovalbumin, phosvitin and κ-casein using a ultra-violet transilluminator. Furthermore, Fura 2 pentapotassium salt stain is able to carry out continuative double detection of total proteins and phosphoproteins on the same gel.8-Quinolinol can form ternary complexes in the gel matrix contributed by the affinity of Al3+ to the phosphate groups on the proteins and due to the metal chelating property of 8-Quinolinol, exhibiting strong fluorescence in ultraviolet light. This method can visualize as little as 4-8 ng of α-casein and β-casein, 16-31 ng of ovalbumin and κ-casein within 70 min.Five novel methods appear to be effective tools considering their simple, rapid and inexpensive procedures.