In this study, cellulose acetate (CA)/poly-(γ-glutamic acid) (γ-PGA)/TiO2 fibrous membrane were prepared byelectrospinning. The wettability of the fibrous membrane was studied by using water contact angle measurements. Scanningelectron and thermogravimetric analyses were carried out to determine the microstructure of the fibers and the thermalstability of the membrane. A study was conducted on the corn seedlings grew of the CA/γ-PGA/TiO2 fibrous membranebasedagricultural film in soil at room temperature. Maize plant height, leaf area and SPAD (Soil and plant analyzerdevelopment) were investigated when covered with a CA/γ-PGA fibrous membrane and a CA/γ-PGA/TiO2 fibrousmembrane. In a simultaneous fashion, water absorption and degradation rate were also studied. Our study highlights thepotential use of these fibrous membranes for mulching film applications. The fibrous membrane may also find potentialapplication in food packing materials, facial masks and antimicrobial films of wound dressing.