Three-dimensional (3D) printing technology provides an effective alternative for tissue engineering scaffold. Inthis paper, a composite scaffold composed of polylactic acid (PLA) film and 3D printing scaffold was fabricated which couldprovide structural and mechanical support for tissue application. The effects of PLA film/3D printing composite scaffold onscaffold morphology, average diameter, pore diameter, mechanical properties, themal conductivity, electrical conductivity,and surface hydrophilicity were characterized. PLA film/3D printing composite scaffold had larger thickness, bettermechanical and thermal conductivity properties in comparison with pure PLA film and 3D printed scaffold. These resultssuggested that composite scaffold with relatively good overall performance could be a potential candidate for tissueengineering application.