Protective mechanism of fdft1 in steroid hormone synthesis pathway in SD rats with acute hypoxic injury
- Resource Type
- Article
- Authors
- Xue Lin; Haiyan Wang; Xiaoyan Pu
- Source
- Genes & Genomics, 42(11), pp.1319-1326 Nov, 2020
- Subject
- 생물학
- Language
- English
- ISSN
- 2092-9293
1976-9571
Background The acute hypoxic injury caused by the plain population entering the plateau in a short period of time hasbecome the main cause of endangering the health of the people who rush into the plateau. Objective The study aimed to identify the key genes which participate in resisting the acute hypoxic injury in SD Rats bytranscriptomic profile analysis. Methods 48 Sprague Dawley (SD) male rats were enrolled and randomly divided into four groups (0h, 24h, 48h, 72h) andhoused in hypobaric hypoxia chamber with altitude 6000m for different periods of time to make them acute hypoxic injury. The transcriptomic profile of the lung tissue of the rats was analysed by RNA second-generation sequencing combined withbioinformatics analysis. Results The results of GO and KEGG function classification analysis revealed that the differential expression genes enrichedin steroid hormone synthesis pathway especially in 48h group compared to F0 group. Further analysis revealed that FarnesylDiphosphate Farnesyl Transferase 1 (fdft1) gene encoding a rate-limiting enzyme in steroid hormone synthesis pathway wassignificant differently expressed between the groups. The expression levels of fdft1 gene were further verified by RT-PCRand Western-blot methods. Conclusions The results suggest that fdft1 gene plays an important role in responding to acute hypoxic injury by regulatingsteroid hormone biosynthesis.