With the application and popularization of computer technology and internet, the electronic commerce industry and tourism industry has been closely connected. E-commerce not only can promote the tourism industry, but also can promote the innovation of the management mode. Construction and management of tourism websites in China is moving towards a good trend. In order to use the resources of tourism website more effectively, this paper uses the selected questionnaire which is focus on the method of R Muhtaseb, K Lakiotaki, N Matsatsinis(2012), to study the usability of several large domestic online travel agency(Ctrip, Lvmama, MangoCity and 17U.cn). In the course of the study, mainly to carry out the test of the questionnaire reliability, and on this basis, we had analysed the basic information of the questionnaire and data after completed three tasks。Then suggestions are given on the basis of a comparison and analysis of the clooected data. With observation, analysis and interpretation of the sample of the type of life and industry, science and technology and humanity in the background of the Internet and the public leisure time, this paper is a study of the cultural view, the behavior theory and the industry development. The authors hope that this study can have some benefits on the construction of tourism websites and the promotion of electronic commerce of cultural industry in China.