Nowadays, the development and application of new technology have a positive impact on the enterprise's operating efficiency and internal control management. In order to explore how firm informationization affects audit fee, we use OLS regression model to examine the relationship between the informationization and audit fee. At the same time, we introduce internal control disclosure and its five elements to explore their moderating effects on the relationship between informationization and audit fee. This article selects the data of Chinese listed companies from 2011 to 2014. Through empirical analysis, we find that informationization of firm has a significantly positive effect on audit fee. Furthermore, internal control disclosure has a significant negative moderating effect on the relationship between informationization and audit fee, but the five elements of internal control disclosure have different moderating effects on the relationship between informationization and audit fee. This study extends literatures on informationization and provides practical suggestions for informationization of companies.