Rhizosphere soils of the 6 tea plants with different ages were collected, and soil autotoxicity and effects of soil to endogenous hormone of tea seedlings were analyzed. The results of bioassays related to soil autotoxicity showed that inhibition rates of the 6 soil extraction to root length of receptors raise as soil age increased, especially after 9 years, which had increased significantly. Analysis results on content of endogenous hormone in tea seedling leaf showed that when the 6 tea soil age exceeded 9 years, inhibitory rate of soil to IAA and ZR, and promoting rate of soil to ABA to rise significantly as soil age increased. Correlation analysis results showed that autotoxity was positive with planting years of tea soil and contents of ABA in the leaf significantly. In conclusion, for the 6 tea cultivars, soil autotoxity increased with the increase of tea soil age, and controlled contents of endogenous hormone in tea seedling leaves. This confirmed soil autotoxity is an important aspect in the formation of continuous cropping obstacles in tea plantation.