In order to explore the traditional highway tunnel electromechanical operation and maintenance mode to the intelligent operation and maintenance mode upgrade application, digital twin technology and highway tunnel electromechanical equipment operation and maintenance of the depth of the fusion of possible. This paper first analyzes the demand pain points of highway tunnel operation and management and the advantages of digital twin technology integration, collects high precision maps, facilities and equipment points, CAD and other multi-source data, establishes the tunnel scene road model, electromechanical equipment three-dimensional model and so on, constitutes the tunnel digital model asset library. Secondly, the tunnel digital twin dynamic assemblage base is constructed to ensure high-precision 1:1 restoration and realize adjustment and editing following the real physical environment. And the lOT sensing technology is integrated into the digital twin base, utilizing information access such as mechanism model, real-time sensing data, historical data, etc., to form a multi-dimensional, multi-scale tunnel mechanical and electrical equipment intelligent operation and maintenance architecture system based on the digital twin, and to build a highway tunnel electromechanical equipment operation and maintenance platform based on the digital twin, which provides support for the state assessment, fault early warning, and operation and maintenance decision-making for mechanical and electrical equipment in the real tunnel environment. Finally, the research results have been successfully applied in Niu Tingling Tunnel in Lishui City, Zhejiang Province, the average fault discovery time is reduced by 5min, and the efficiency of automatic O&M dispatching is increased by 33%, etc. This verifies the effectiveness of digital twin technology in enhancing the intelligence of tunnel O&M management, and it serves as a reference for the promotion of intelligent O&M mode of digital twin technology in the highway in terms of high efficiency and low cost.