Commercial portable electrochemical sensors have rapidly developed in recent years, the decreased detection accuracy caused by contamination is still a huge challenge. Here, an electrode decontamination strategy based on microrobots powered by ultrasound was designed to realize the electrode regeneration. Basic configurations about the manipulation-integrated sensor platform (MISP) are proposed to enable the dual functions of electrochemistry detection and electrode regeneration. Two different vibration modes of the MISP are used to excite enrichment and separation, respectively. Subsequently, we designed and characterized the cleaning microrobots and discussed its role in electrode regeneration. At last, the effects of electrode regeneration are verified with H 2 O 2 sensors. The active method mentioned in this work based on microrobots and acoustic manipulation paves the way for electrode regeneration during detection, enabling electrochemical sensors to be more favorable for myriad applications in future.