Electrically Facilitated Transport of Proteins through Glass Nanopores: Electroosmosis versus Electrophoresis
- Resource Type
- Conference
- Authors
- Yao, Xun; Li, Hongluan; Wang, Shichang; Xu, Wei; Ma, Jian; Mo, Jingwen
- Source
- 2023 IEEE 18th International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) Nano/Micro Engineered and Molecular Systems (NEMS), 2023 IEEE 18th International Conference on. :193-196 May, 2023
- Subject
- Bioengineering
Components, Circuits, Devices and Systems
Engineered Materials, Dielectrics and Plasmas
Photonics and Electrooptics
Robotics and Control Systems
Proteins
Electric potential
Biotechnology
Nanoelectromechanical systems
Bovine
Glass
Molecular biophysics
glass nanopore
protein
electroosmosis
electrophoresis
- Language
- ISSN
- 2474-3755
Nanopores are powerful tools for single molecule detection in biotechnology. A critical challenge of nanopore detection is the large translocation speed of proteins across the nanopore. In this work, we used glass nanopores made by laser-assisted capillary pulling to investigate the transport characteristics of bovine serum albumin (BSA) driven by electrophoretic or electroosmotic flow respectively. These two flow modes are achieved by modulating the direction of the applied electric field. The experimental results demonstrate that the translocation speed of BSA through glass nanopores driven by electroosmotic flow is significantly slower than that driven by electrophoretic flow. A lower translocation speed is more favorable, which allows more events to be detected and provides more insights into biomolecular information.