Detection of short peptides and dimers using silicon nitride nanopores
- Resource Type
- Conference
- Authors
- Bian, Peifu; Zou, Lichun; Wang, Haiyan; Sha, Jingjie
- Source
- 2023 IEEE 18th International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) Nano/Micro Engineered and Molecular Systems (NEMS), 2023 IEEE 18th International Conference on. :23-27 May, 2023
- Subject
- Bioengineering
Components, Circuits, Devices and Systems
Engineered Materials, Dielectrics and Plasmas
Photonics and Electrooptics
Robotics and Control Systems
Proteins
Drugs
Nanoelectromechanical systems
Peptides
Hydrogen
DNA
Silicon nitride
Soild-state nanopore
silicon nitride
short peptide
mass spectromentry
- Language
- ISSN
- 2474-3755
Solid-state nanopores have previously been applied to the recognition and detection of DNA and proteins as a powerful tool for single-molecule detection. Here, we use silicon nitride nanopores to identify short peptides of six amino acids in length and their dimers. The experimental results show that the blocking current of the dimer is close to twice as large as that of the monomer. Due to the lower charge, the probability of the dimer passing through the pore at voltages below 100mV is low. In addition, the results of the mass spectrometry assay provide evidence for a translocation event of the short peptides and their dimers.