An optogenetic tool to inhibit RhoA in Drosophila embryos
- Resource Type
- article
- Authors
- Hanqing Guo; Michael Swan; Bing He
- Source
- STAR Protocols, Vol 4, Iss 1, Pp 101972- (2023)
- Subject
- Cell Biology
Developmental biology
Genetics
Microscopy
Model Organisms
Science (General)
Q1-390
- Language
- English
- ISSN
- 2666-1667
Summary: We describe a protocol for optogenetic inhibition of the small GTPase Rho1 (RhoA) in Drosophila embryos, which allows rapid and spatially confined inactivation of Rho1 and Rho1-mediated actomyosin contractility. We provide step-by-step instruction for optogenetic manipulations of Drosophila embryos using confocal and multiphoton imaging systems. This tool is useful for determining the site- and stage-specific function of Rho1 in Drosophila embryos and for studying the immediate tissue response to acute elimination of cellular contractility.For complete details on the use and execution of this protocol, please refer to Guo et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.