Objective To explore the distribution of T lymphocyte subsets in peripheral blood of healthy adults from Chashan Town of Dongguan in Guangdong Province. Methods The venous whole blood samples were collected from 827 healthy adults. The percentage of CD3+ T cells, CD4+ T cells, CD8+ T cells, CD4+CD8+ double positive (DP) T cells, and regulatory T(Treg) cells and CD4+/CD8+ ratio were determined by flow cytometry. Results The percentage of CD3+ T cells, CD4+ T cells, CD8+ T cells, CD4+/CD8+ ratio, DP T cells and Treg cells were determined as 40.5%-75.2%, 35.4%-68.9%, 27.4%-61.8%, 0.6-2.4, 0.2%-2.7% and 2.4%-6.0%, respectively. Significant differences were observed in the distribution of lymphocyte subsets regarding age and gender (all P < 0.05). Conclusion The distribution of T lymphocyte subsets in healthy adults from Chashan Town of Dongguan in Guangdong Province was successfully analyzed, providing valuable reference for clinicians in this region.