Introduction: Central line-associated bloodstream infections (CLABSIs) are important causes of mortality and morbidity in pediatric intensive care units (PICUs). This study aimed to investigate the effect of a 2% chlorhexidine gluconate (CHG) bath on CLABSI. Materials and Methods: Skin bathing was performed every other day with 2% CHG in patients as of 25.04.2019. The frequency of CLABSI pre and post-intervention was evaluated. Results: A total of 226 patients were included in this study, 111 patients before CHG and 115 patients after CHG. CLABSI rates before and after using CGH were 10.11 and 5.52, respectively. The relative risk of CLABSI frequency during CHG bathing was compared to the control period and resulted in a significant reduction (13.0%, p< 0.0001, 95% CI= 2.5-4.2%). Conclusion: Adding a 2% CHG bath every other day to CLABSI precaution bundles may be an effective strategy for reducing the frequency of CLABSI in PICUs.