Objective: To compare the IVF pregnancy group andspontaneous pregnancy group in terms of PAPP-A and free β-hCGMaterials and methods: Among the pregnant women who applied fordouble test screening between September 2017 and September 2018, 42 singletonpregnancies that were pregnant as a result of IVF (in vitro fertilization)constituted our study group where 109 singleton pregnancies whose age rangewere matching the IVF pregnacies but were pregnant spontaneously without anyassisted reproductive technologies (ART) remained in our control group. Thedemographic data and pregnancy outcomes of both pregnancies were similar.Findings:The mean PAPP-A (pregnancyassociated plasma protein-A) was found in the control group (2.82 mlU / ml;0.97 MoM), while in the study group 2.41 mlU / ml, 0.94 MoM). Free β-hCG(beta-human chorionic gonadotropin) values were found as 46.44, ng / ml;1.21MoM, 53.79ng / ml in control and study groups respectively. The mean PAPP-Aof the IVF pregnancy group was lower than the spontaneous pregnancy group, andthe mean β-hCG was higher than the spontaneous pregnancy group, but thesedifferences were not statistically significant (p> 0.005). Although notstatistically significant; In terms of trisomy 21 we evaluated the study groupas higher risk than the control group (p> 0.05).Results: The mean of PAPP-Aand free β-hCG values are different in IVF and spontaneous pregnancies, andthese differences in biochemical parameters affect the risk of trisomy 21.