CE to monitor endotoxins by protein complexation
- Resource Type
- Authors
- Stellan Hjertén; Anikó Kilár; Ferenc Kilár; Ildikó Kustos; Béla Kocsis
- Source
- ELECTROPHORESIS. 27:4188-4195
- Subject
- Lipopolysaccharides
Salmonella
Chromatography
Chemistry
Bacterial Toxins
Clinical Biochemistry
Uv absorption
Electrophoresis, Capillary
Proteins
Shigella sonnei
medicine.disease_cause
Biochemistry
Analytical Chemistry
Hemoglobin complex
Hemoglobins
Electrophoresis
Qualitative analysis
medicine
Electrophoretic mobilities
Hemoglobin
- Language
- ISSN
- 1522-2683
0173-0835
A new CE method for fast and efficient analysis of bacterial endotoxins (lipopolysaccharides) is described. It is based on the strong interaction between proteins and endotoxins. The UV absorption of the protein component in the complex is used for the detection. The electrophoretic mobility of the complex hemoglobin/endotoxin can be employed for qualitative analysis of the endotoxin. For instance, the structural differences between "smooth" and "rough" lipopolysaccharides from Salmonella minnesota (wild-type), Salmonella minnesota R595 and Shigella sonnei R562H are reflected in the electrophoretic mobilities of their hemoglobin complex.