Additional file 1: Table S1. Heterologous genes, strains, plasmids, and primers used in this study. Table. S2. Synthesized gene sequences. Table S3. Level of factors chosen for the central composite design response surface methodology and ANOVA analysis of the model obtained for optimal medium. Table S4. Isopropanol yield results optimal YPD medium for CCD-RSM. Figure S1. YLnphT7IPA strain was cultured in 5 L fermenter using glucose as carbon source.