Metabotropic glutamate receptor mGluR2/3 immunoreactivity in the mouse superior colliculus
- Resource Type
- Authors
- Chang-Jin Jeon; Min-Ah Kim
- Source
- NeuroReport. 10:1341-1346
- Subject
- Calbindins
Superior Colliculi
General Neuroscience
Superior colliculus
Enucleation
Immunocytochemistry
Glutamate receptor
Fluorescent Antibody Technique
Nerve Tissue Proteins
Retinal
Anatomy
Biology
Receptors, Metabotropic Glutamate
Immunohistochemistry
Molecular biology
Calbindin
Mice, Inbred C57BL
Mice
chemistry.chemical_compound
S100 Calcium Binding Protein G
chemistry
Calbindin 1
Metabotropic glutamate receptor
Hepatic stellate cell
Animals
- Language
- ISSN
- 0959-4965
We have studied the distribution of mGluR2/3 in the mouse superior colliculus (SC) with antibody immunocytochemistry and the effect of enucleation on this distribution. We also compared this labeling to that for calbindin D28K. Anti-mGluR2/3-immunoreactive (IR) cells formed distinctive laminar patterns within the lower optic and upper intermediate gray layers. By contrast, anti-calbindin D28K-IR cells formed obvious laminar patterns in three layers: one within the zonal and upper superficial gray layers, a second within the optic and intermediate gray layers, and the third within the deep gray layer. The distribution of mGluR2/3-IR cells thus matches the second layer of calbindin D28K cells. Two-color immunofluorescence revealed that more than half (52.5%) of mGluR2/3-IR cells were also labeled with antibody to calbindin D28K. The majority of mGluR2/3-IR cells were small to medium-sized round/oval or stellate cells. Immunoreactivity for mGluR2/3 was clearly reduced in the contralateral SC following unilateral enucleation. The present results show that mGluR2/3 has a unique cellular sublaminar organization in SC that includes some calbindin D28K-IR cells. The effects of enucleation suggest that the retinal projection may control the expression of mGluR2/3 in some cells in the mouse SC.