Additional file 1 of Metabolic mechanism of astaxanthin biosynthesis in Xanthophyllomyces dendrorhous in response to sodium citrate treatment
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- Pan, Xueshan; Li, Tonggang; Wang, Baobei; Qi, Shuhua; Yang, Dandan; Huang, Zheng; Gao, Renfei; Li, Jingyan; Ling, Xueping; Lu, Yinghua
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Additional file 1: Figure S1. Effect of 2 g/L Na-citrate addition at different times on the growth and astaxanthin production of X. dendrorhouos. (A) Biomass (g/L); (B) Carotenoids titer (mg/L); (C) Astaxanthin titer (mg/L); (D) Astaxanthin content (mg/g). The cells were grown in a 250-mL Erlenmeyer flask containing 50 mL fermentation medium, with the temperature maintained at 22°C and the stirring speed at 200 rpm. Values are mean ± standard deviation of three independent experiments. Figure S2. Effect of different Na-citrate concentrations at 24 h on the growth and astaxanthin production of X. dendrorhouos. (A) Biomass (g/L); (B) Carotenoids titer (mg/L); (C) Astaxanthin titer (mg/L); (D) Astaxanthin content (mg/g). The cells were grown in a 250-mL Erlenmeyer flask containing 50 mL fermentation medium, with the temperature maintained at 22°C and the stirring speed at 200 rpm. Values are mean ± standard deviation of three independent experiments. Figure S3. Na-citrate regulates the protein content in X. dendrorhouos. fold change is the ratio of the protein content of the control group to the Na-citrate group. Table S1. Gene-specific primers used for RT-qPCR; F: Forward; R: Reverse