Surface Plasmon Resonance Study of SKBR3 Antibody and Antigen Interaction by Using Spectroscopic Ellipsometry
- Resource Type
- Authors
- Min-Ah Woo; Yunbog Kim; Myunghaing Cho; Dongryul Jeon
- Source
- Journal of the Korean Physical Society. 53:1419-1423
- Subject
- Materials science
biology
Atomic force microscopy
education
General Physics and Astronomy
Antigen-antibody interaction
Nuclear magnetic resonance
Antigen
SKBR3
biology.protein
Spectroscopic ellipsometry
Breast cancer cells
Surface plasmon resonance
Antibody
skin and connective tissue diseases
- Language
- ISSN
- 0374-4884
Using spectroscopic ellipsometry as a surface plasmon resonance (SPR) detector, we studied the antibody-antigen interaction of the Her-2/neu protein from SKBR3 breast cancer cells. Chips were prepared by immobilizing the HER2 antibody on a linker-coated Au surface and the SPR was measured after dipping the chip in SKBR3 bu er and drying. The shifts of the SPR peak at each step of the chip preparation and the corresponding morphology change in the atomic force microscopy images suggested that this method could be useful for the diagnosis of cancerous cells in vitro.