Lesson of the month: novel method to quantify neutrophil uptake in early lung cancer using SPECT-CT
- Resource Type
- Authors
- Farahi, Neda; Gillett, Daniel; Southwood, Mark; Rassl, Doris; Tregay, Nicola; Hill, Uta; Preston, Stephen Denis; Loutsios, Chrystalla; Lok, Laurence Si Chong; Heard, Sarah; Buscombe, John; Rintoul, Robert Campbell; Peters, A Michael; Summers, Charlotte; Chilvers, Edwin R
- Source
- Subject
- lung cancer
Chest clinic
neutrophil biology
imaging/CT MRI etc
digestive system
3. Good health
- Language
Neutrophils play an important role in the lung tumour microenvironment. We hypothesised that radiolabelled neutrophils coupled to single-photon emission CT (SPECT) may non-invasively quantify neutrophil uptake in tumours from patients with non-small cell lung cancer. We demonstrated increased uptake of radiolabelled neutrophils from the blood into tumours compared with non-specific uptake using radiolabelled transferrin. Moreover, indium-111-neutrophil activity in the tumour biopsies also correlated with myeloperoxidase (MPO)-positive neutrophils. Our data support the utility of imaging with In-111-labelled neutrophils and SPECT-CT to quantify neutrophil uptake in lung cancer.