Highlights: [•] We firstly verified that andrographolide sodium bisulfate (ASB) could suppress HK-2 cell growth and proliferation within the concentration ranging from 7mmol/L to 57mmol/L. [•] We firstly found that ASB could induce ROS generation abundantly in HK-2 cells, activate JNK signaling pathway, and further induce cell apoptosis via mitochondria dependent-caspase pathway. [•] Interestingly, ASB could induce HK-2 cell autophagy as well in the concentration of 14 and 29mmol/L respectively, which maybe a result of Beclin-1 upregulating. [•] Our findings provided a novel pathway for the study of mechanism underlying ASB-induced nephrotoxicity. [ABSTRACT FROM AUTHOR]