Deciphering the microbial community tolerance mechanism and alteration of antibiotic resistance genes during chloramphenicol wastewater treatment.
- Resource Type
- Article
- Authors
- Zhou, Jia; Chen, Yan; Li, Wan-Xia; Qu, Jian-Hang; Chen, Tian; Wang, Yi-Ping; Geng, Ning-Yu
- Source
- International Biodeterioration & Biodegradation. Mar2023, Vol. 178, pN.PAG-N.PAG. 1p.
- Subject
- *DRUG resistance in bacteria
*CHLORAMPHENICOL
*WASTEWATER treatment
*ECOSYSTEM health
*GENES
*MICROBIAL communities
- Language
- ISSN
- 0964-8305
Excessive use of chloramphenicol (CAP) and its residues has emerged as a potential threat to human health and ecological safety. It is challenging to reveal the key microbial species in the CAP biodegradation process and interactions of the microbial community with the antibiotic resistance genes (ARGs). The present study sought to investigate the interactions of microbial community with ARGs during the sequential batch reactor (SBR) treatment of CAP wastewater to explore the potential antibiotic resistance mechanisms. The treatment showed excellent performance with 95.5% CAP removal efficiency based on the core genera of Candidatus Competibacter and Hydrogenophaga with strong antibiotic resistance and adaptability to the increasing CAP concentration in the influent. Meanwhile, Candidatus Competibacter and Hydrogenophaga were significantly associated with the primary CAP resistance genes of cml_e3 and cml_e8, which developed resistance against CAP pressure through efflux pump mechanism. Furthermore, the two CAP efflux pumps genes (floR and cmlA) were stimulated at low CAP level (0.01–0.1 g L−1) while suppressed at high CAP level (0.2 g L−1). This study emphasizes that SBR is an eco-friendly strategy to remove CAP by enriching the core genera of Candidatus Competibacter and Hydrogenophaga through the efflux pump mechanism to against the CAP pressure. Interactions of microbial community with antibiotic resistance genes. [Display omitted] • Chloramphenicol could be effectively removed with 80.0%–95.5% removal efficiency. • Chloramphenicol promoted the enrichment of Candidatus Competibacter and Hydrogenophaga. • High concentration of chloramphenicol significantly inhibited the increase of floR and cmlA. • Efflux pump mechanism was primary for bacterial resistance to chloramphenicol. [ABSTRACT FROM AUTHOR]