Using activated charcoal to remove substances interfering with the colorimetric assay of inorganic phosphate in plant extracts.
- Resource Type
- Article
- Authors
- Dayrell, Roberta L. C.; Cawthray, Gregory R.; Lambers, Hans; Ranathunge, Kosala
- Source
- Plant & Soil. Jul2022, Vol. 476 Issue 1/2, p755-764. 10p. 1 Color Photograph, 1 Chart, 3 Graphs.
- Subject
- *ACTIVATED carbon
*MALACHITE green
*PLANT extracts
*PHOSPHATES
*ACETIC acid
*ADSORPTION capacity
- Language
- ISSN
- 0032-079X
Aim: Organic substances in leaves of several southwest Australian native species interfere with sensitive colorimetric assays and prevent quantification of inorganic phosphate concentration ([Pi]). We aimed to develop a reproducible routine procedure for treating leaf extracts with activated charcoal (AC) to remove interfering substances, allowing the determination of [Pi] by the malachite green spectrophotometric assay. Methods: Leaf extracts of native plants from southwest Australia in 1% (v/v) acetic acid were treated with 10 mg mL−1 acid-washed AC for removal of interfering substances. Standard solutions (0 to 18 μM Pi) with and without AC treatment were compared to quantify Pi loss. A spiking and recovery test was performed to validate the AC treatment. Results: Leaf extracts treated with AC exhibited distinguishable absorbance peaks for the malachite green-orthophosphate complex between 630 and 650 nm, as opposed to untreated samples. The Pi-adsorption by AC represented a relatively larger fraction of [Pi] in solutions at 0–4 μM Pi range and stabilised at higher [Pi] when maximum adsorption capacity of AC reached at 11.7 μg Pi g−1AC. The Pi recovery after AC treatment in spiked samples ranged between 100 and 111%. Conclusion: The AC treatment successfully removed interfering substances from samples but caused Pi loss. Thus, quantification of [Pi] in AC-treated extracts requires sample [Pi] ≥ 6 μM Pi and the use of AC-treated standards. The error of the AC treatment was minor compared with environmental variability of leaf [Pi]. The AC treatment was a reproducible time- and cost-effective method to remove interfering substances from leaf extracts. [ABSTRACT FROM AUTHOR]