5,6-Benzoflavone (1), a new natural product, has been isolated from the EtOH extract of the flowers of Alstonia scholaris (L.) R. Br. (Apocynaceae), along with the known compound dicoumarol (2). Phytochemical studies on the fruits also provided bergenin (3), 9,19-cycloart-23-ene-3,25-diol (4), 11-oxo-α-amyrin acetate (5), and marsformoxide B (6). Compounds 2–6 have been isolated for the first time from the genus Alstonia. Their structures were elucidated by spectroscopic techniques, including two-dimensional NMR. Biological screening of the isolates revealed the significant urease inhibitory potential of compounds 1–6 while 4–6 also showed significant antibacterial activity against S. typhi and P. mirabilis. [ABSTRACT FROM AUTHOR]