The aim of this research is to analyze anxiety, knowledge, and belief toward e-learning acceptance, especially by science teachers in high schools in Aceh, Indonesia. About 117 teachers were randomly selected for this study. A questionnaire was used to collect data, and analyzed using the Likert scale. The results indicate that most teachers view the use of e-learning in teaching science to be positive. Despite the fact that only half of them are knowledgeable in the use of media and have concerns about some negative effects of e-learning on students, they still believed in the usefulness of e-learning in science education. Hence, more related research is recommended to further corroborate the findings of this study. [ABSTRACT FROM AUTHOR]