Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation.
- Resource Type
- Article
- Authors
- Chen, Rong; Wei, Qiuhui; Wei, Xin; Liu, Yuheng; Zhang, Xiaomin; Chen, Xiabin; Yin, Xiaopu; Xie, Tian
- Source
- PLoS ONE. 11/18/2020, Vol. 15 Issue 11, p1-15. 15p.
- Subject
- *NICOTINAMIDE adenine dinucleotide phosphate
*COFACTORS (Biochemistry)
*MICROWAVES
*CALCIUM alginate
*IRRADIATION
*CATALYSTS
- Language
- ISSN
- 1932-6203
One of the challenges in biocatalysis is the development of stable and efficient bi-enzymatic cascades for bio-redox reactions coupled to the recycling of soluble cofactors. Aldo-keto reductase (LEK) and glucose dehydrogenase (GDH) can be utilized as the NADPH recycling system for economic and efficient biocatalysis of (R)-4-chloro-3-hydroxybutanoate ((R)-CHBE), an important chiral pharmaceutical intermediate. The LEK and GDH was efficiently co-immobilized in mesocellular siliceous foams (MCFs) under microwave irradiation (CoLG-MIA). while they were also co-immobilized by entrapment in calcium alginate without MIA as control (CoLG-CA). The relative activity of CoLG-MIA was increased to 140% compared with that of free LEK. The CoLG-MIA exhibited a wider range of pH and temperature stabilities compared with other preparations. The thermal, storage and batch operational stabilities of microwave-assisted immobilized LEK-GDH were also improved. The NADPH recycling system exhibited the potential as the stable and efficient catalyst for the industrial preparation of (R)-CHBE. [ABSTRACT FROM AUTHOR]