Amplified detection of genome-containing biological targets using terminal deoxynucleotidyl transferase-assisted rolling circle amplification.
- Resource Type
- Article
- Authors
- Du, Yi-Chen; Zhu, Yan-Jun; Li, Xiao-Yu; Kong, De-Ming
- Source
- Chemical Communications. 1/18/2018, Vol. 54 Issue 6, p682-685. 4p.
- Subject
- *AMPLIFICATION reactions
*TRANSFERASES
*MOLECULAR structure of DNA polymerases
- Language
- ISSN
- 1359-7345
We proposed a terminal deoxynucleotidyl transferase (TdT)-assisted rolling circle amplification (RCA) strategy for the amplified detection of genome-containing biological targets. The synergetic signal amplification of DNase I-mediated genomic DNA fragmentation and subsequent RCA allow the sensing platform to have extraordinarily high sensitivity. [ABSTRACT FROM AUTHOR]