Aim: Angiogenesis aims to counteract the progression of atherosclerotic disease. The aim of this study is to characterize the role, safety and efficacy of the local therapy that must be associated with this procedure in a dedicated nursing service. Method: From 2014 to 2022, 400 operations were performed in our center for obstructive arterial disease of the lower limbs, caused by PAD. In 30 cases they were patients in stage I (7.7%), 244 operations in stage I 2 (61%), 32 operations for arterial disease in stage II 4 (8%) and in 76 cases in stage III 5 (19 %), 19 patients with a mean age of 74.75 (SD 9.4) were considered nonrevascularizable (4.8%). 48 treatments were performed (2.5 treatments/patient, min2/max 4). This was a first therapeutic approach in 55%, while it was proposed as a therapeutic alternative in patients who had already undergone previous operations and/or revascularization attempts in 45%. After treatment, all patients with trophic lesions underwent periodic medications with checkups in the dedicated nursing clinic. Results: In the 19 non-revascularizable patients or with failure of revascularization therapy that were considered, limb salvage was observed in 14/19, 73.6%, major amputation in 1/19 case (5,2%), and in 4/19 cases (21.2%) unrelated death. Conclusions: Treatment with mononuclear cells by selective filtration is an effective procedure, in a selected patients. The association of advanced medications, carried out at the dedicated service, has made it possible to obtain the good results obtained.