OP118 GENOMIC BIOMARKERS PROVIDE A DEEPER UNDERSTANDING FOR RECURRENT PRESSURE ULCERS...European Wound Management Association (EWMA) Conference, May 3-5, 2023, Milan, Italy
- Resource Type
- Academic Journal
- Authors
- Bogie, Kath; Graves, Letitia; Chan, E. Ricky; Schwartz, Katie; Henzel, M. Kristi; Richmond, Mary Ann; Alvarado, Nannette; Shiff, Josie; Galea, Marinella; Toth, Anna; Olney, Christine
- Source
- Journal of Wound Management Jul2023; 24(2): 455-455. (1/2p)
- Subject
- Language
- English
- ISSN
- 2788-5771
Aim: The study is investigating the enigma of why some high risk individuals stay pressure ulcers (PU) free whilst others experience a continuous cycle of recurring PU, leading to long periods of bedrest and reduced quality of life. Genomic biomarkers that may facilitate earlier identification of individuals at the highest risk for recurrent PU are being investigated. Method: RNA sequencing of processed whole blood samples has been carried to examine repeated measures of the transcriptome activity of 60 persons with complete or incomplete SCI (AIS A-D) and known PU history. Samples were collected at 6-12 month intervals for all participants. RNA was isolated and mixed with Deoxyribonuclease (DNase) to reduce the amount of genomic DNA. Quality control was carried out using the Qubit™ RNA broad range kit and normalized to 50ng/uL prior to sequencing using the NovaSeq 6000 (Illumina) system. Sequence data was analysed using iPathwayGuide (Advaita Bioinformatics). Results / Discussion: A robust subset of 25 active genes that are differentially up- or down-regulated between persons with or without recurrent PU has been identified. Persons with recurrent PU have upregulated activity in genes involved in the pathways in biological senescence and downregulated activity in pathways directly involved in antimicrobial protection. Conclusion: The huge impact of recurrent PU on quality of life remains challenging. 21st century approaches to an age-old problem are providing greater understanding of the role of genomic biomarkers for increased risk for recurrent PU. An ongoing multisite repeated measures study is providing further insights into multi-omic risk for recurrent PU.