Effect of glucose and other sugars on the. beta. -1,4-glucosidase activity of Thermomonospora fusca
- Resource Type
- Journal Article
- Authors
- Source
- Biotechnol. Bioeng.; (United States); 25:12
- Subject
- 09 BIOMASS FUELS GLUCOSE
BIOLOGICAL EFFECTS
GLUCOSIDASE
INHIBITION
CELLOBIOSE
CELLULOSE
CHEMICAL ACTIVATION
ENZYME ACTIVITY
ENZYME INHIBITORS
QUALITATIVE CHEMICAL ANALYSIS
ALDEHYDES
CARBOHYDRATES
CHEMICAL ANALYSIS
DISACCHARIDES
ENZYMES
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
POLYSACCHARIDES
SACCHARIDES 090222* -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
- Language
- English
The cell-associated ..beta..-glucosidase activity of Thermomonospora fusca, strain YX, showed both PNPGase and cellobiase activities. The cellobiase activity was found by HPLC assay to have very low product inhibition, whereas the PNPGase activity was more significantly inhibited. Of the various sugars and sugar analogs tested for inhibition of the PNPGase activity, gluconolactone had the greatest effect. The low product inhibition of the cellobiase activity was further demonstrated by the production of glucose syrups to 20% concentration from both cellobiose and swollen cellulose (Avicel). This characteristic is of practical importance in the development of a commercial process for the production of glucose syrups from cellulose. Growth experiments gave further evidence for the probability of separate enzymes for the PNPGase and cellobiase activities.