This work includes the synthesis of a new ionic liquid (IL) derived from benzisothiazolinon (Bit) by the reaction of benzisothiazolinon with HCl at room temperature. This ionic liquid (BitIL) was characterized using FTIR, 1HNMR, 13C-NMR, and elemental analysis. Furthermore, eggshell membrane protein polymer (ESMP) was used as a natural polymer to prepare its nanocomposite with titanium oxide nanoparticles (TiO2 NPs) (ESMP-TiO2). At the end of the synthesis route, the as-prepared benzisothiazolinon ionic liquid (BitIL) was grafted on the surface of the nanocomposite to synthesis benzisothiazolinon ionic liquid@ eggshell membrane protein polymer–titanium oxide (BitIL@ESMP-TiO2) nanocomposite by the one-pot method. The BitIL@ESMP-TiO2 nanocomposite was characterized using FTIR, XRD, and TEM. Moreover, the kinetics of in vitro release of BitIL from BitIL@ESMP-TiO2 was studied using Korsmeyer and Peppas equation at two different temperatures and pHs. From the results, it was found that the BitIL released from ESMP-TiO2 was about 85%, at 37 °C. Furthermore, the pH = 1.2 (stomach acidity) produced n values of 0.645 and 0.761, indicating the prevalence of anomalous type, whereas the pH = 7.2 (blood acidity) produced quasi-Fickian type diffusion.