The novel hybrid ‘BRS Isis’ and ‘BRS Nubia’ table grapes, with seedless and large berries, respectively, have wide adaptability and pest tolerance, factors that are contributing to the renewal of Brazilian vineyards. However, there is a gap regarding the berry quality of these new cultivars, especially in terms of bioactive compounds and volatile profile. This study aimed to characterize the physicochemical parameters as well as phenolic and volatile compounds of ‘BRS Isis’, ‘BRS Nubia’, and ‘Italia’ table grapes grown in two terroirs. The table grapes were analyzed according to their physical and chemical properties, as well as their phenolic, antioxidant, and volatile compounds. ‘BRS Nubia’ presented berries larger in dimensions and mass and, like the ‘Italia’, a more rounded shape, while the ‘BRS Isis’ presented more elongated and firmer berries, regardless of the maturation index. ‘BRS Isis’ and ‘BRS Nubia’ are rich in bioactive compounds, especially ‘BRS Nubia’. On the other hand, the traditional ‘Italia’ was more aromatic in relation to the other cultivars, which presented an aroma characterized as neutral. This study reported relevant information on table grapes produced in temperate climate, especially for the new ‘BRS Nubia’ and ‘BRS Isis’, which are recent alternatives for production and consumption to the traditional ‘Italia’.