Background: Successful resolution of vesicoureteric reflux (VUR) via endoscopic methods has been achieved in the last two decades. After investigating multiple agents, Deflux is being used at most of the centers. We have studied the safety and efficacy of Deflux in our patients having VUR with complex urogenital anomalies and reviewed the literature.Methods: We have retrospectively collected the data of 28 children with the diagnosis of VUR, treated endoscopically over a period of 3 years. The data were collected from the hospital records and analyzed. The data included demographics, associated anomalies, grades of reflux, number of ureters affected, treatment given, number of sittings required, initial findings of micturating cystourethrograms, follow-up cystogram findings, and details of the procedure done. The data were evaluated for overall success rates in children with associated anomalies.Results: Among the 28 patients, 22 children with 33 affected ureter units having associated anomalies (secondary VUR) were included in the study, with 18 boys and 4 girls. Six children having primary VUR and not associated with other congenital anomalies were excluded. The reflux was unilateral in 10 (45.45%) children and bilateral in 12 (54.54%) children. Reflux was Grade II in 11 (33.33%), Grade III in 9 (27.27%), Grade IV in 2 (6.06%), and Grade V in 11 (33.33%) of the 33 ureters. After the first injection of Deflux, complete resolution was observed in 28 (84.84%) ureters and 5 (15.15%) had partial resolution of the VUR at 3-month follow-up. At 1 year of the first injection, complete resolution was achieved in 32 (96.96%) ureters and 1 (3.03%) ureter still had persistent Grade IV reflux, for which he had undergone ureteric reimplantation.Conclusions: We have observed that by endoscopic method reflux resolution was achieved in 84.84% after 1st and 96% children after 2nd injection. So based on our experience, we recommend the Deflux procedure as the primary treatment modality for VUR even in patients with associated complex urological anomalies and bladder dysfunction.