Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method
- Resource Type
- article
- Authors
- Rubiano, Claudia C; Wasserman, Moises
- Source
- Memórias do Instituto Oswaldo Cruz. July 2003 98(5)
- Subject
- Plasmodium
malaria
telomerase
assay
nonradioactive
- Language
- English
- ISSN
- 0074-0276
A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10² to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells.