Elucidating the Mode of Action of Marine Natural Products through an Immunoaffinity Fluorescent (IAF) Approach
- Resource Type
- article
- Authors
- La Clair, James J.; Fenical, William; Costa-Lotufo, Leticia V.
- Source
- Journal of the Brazilian Chemical Society. August 2016 27(8)
- Subject
- marine natural product
mode of action
drug discovery
target identification
confocal microscopy
- Language
- English
- ISSN
- 0103-5053
Understanding the target and mode of action (MOA) of small molecules activity has become a critical feature in guiding the transition between drug discovery and clinical evaluation. While classically placed at the latter stages of a discovery program, we now describe how addressing the mode of action of natural products at the early stages of a program provides an important vehicle to inform the drug discovery process. In this review, we outline a streamlined cellular and molecular biological system and explore its utility through a series of four cases studies. We believe that this approach offers critical lessons to guide future drug discovery programs.