The goal of the current study is to assess the antitumor mechanism by combination of Angelica gigas and Torilis japonica Ethanol Extracts (AT) in prostate cancers. Here, AT reduced the viability and the number of colonies in LNCaP prostate cancer cells. Furthermore, AT induced G1 phase arrest and decreased the protein expression of Cyclin D1, Cyclin E, cdk2 and increased p27 in LNCaP cells. Also, AT significantly decreased expression of PSA level and AR at mRNA and protein levels in LNCaP cells. Interestingly, AT attenuated the expression of AR, PSA and Wnt-3a and also reduced AR and PSA stability in LNCaP cells. Conversely, dihydrotestosterone (DHT) reversed the ability of AT to reduce AR and PSA, since AR and PSA were upregulated by DHT in a time-dependent manner. Notably, AT disrupted the protein-protein interaction as well as reduced nuclear translocation and fluorescent expression of β-catenin and AR in LNCaP cells. Taken together, our findings suggest that combination of Angelica gigas and Torilis japonica suppresses proliferation of LNCaP cells via G1 arrest and inhibition of β-catenin and androgen receptor as a potent anticancer agent.