Microarray probe Tm matching by selective destabilization
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Methods and systems for designing oligonucleotide probes for use in microarray applications are provided herein. The described methods use duplex melting temperature (Tm) matching to destabilize the hybridization oligonucleotide probes to non-target sequences as compared to a target nucleotide sequence. Nucleic acid arrays containing probes selected by the described methods are also described.