PURPOSE OF STUDY: To identify the 3D printed material that most accurately recreates the visual, tactile, and kinesthetic properties of human temporal bone. METHODS USED: This is a prospective qualitative assessment performed at a tertiary care academic institution. 13 otolaryngology residents and 2 otolaryngology attendings with an average of 3.6 years of post-graduate training and 56.5 temporal bone (TB) procedures participated in the study. Each participant performed a partial mastoidectomy on human cadaveric bone then five 3D printed TBs each of a different material. After drilling each unique material, the participant filled out a survey to assess each model’s appearance and physical likeness on a Likert scale from 0 to 10 (0 being least representative and 10 being the most representative). The 3D models were acquired by CT imaging and segmented using 3D Slicer software. SUMMARY OF RESULTS: Polyethylene terephthalate (PETG) had the highest average Likert survey response for feel and appearance, scoring 8.3 (SD=1.7) and 7.6 (SD=1.5), respectively. The remaining plastics scored as follows for feel and appearance, respectively: polylactic acid (PLA) averaged 7.4 and 7.6, acrylonitrile butadiene styrene (ABS) averaged 7.1 and 7.2, polycarbonate (PC) averaged 7.4 and 3.9, and nylon averaged 5.6 and 6.7. CONCLUSIONS: PETG 3D printed temporal bone models offer the most realistic appearance and haptic feedback as compared with PLA, ABS, PC and nylon. PLA and ABS were reliable alternatives that also performed well with both measures.(Table is included in full-text article.)