BACKGROUND:: Interbody fusion is an important method for lumbar spondylolisthesis. The effects of different bone materials require further studies. OBJECTIVE:: To compare the effects of autologous bone, allogeneic bone, bone morphogenetic protein composite bone in patients with lumbar spondylolisthesis. METHODS:: Totally 119 cases of lumbar spondylolisthesis were selected and randomly divided into three groups: autologous bone group (40 cases), allogeneic bone group (41 cases), bone morphogenetic protein composite bone group (38 cases). Intervertebral height and fusion rate were compared after fusion as well as long-term clinical efficacy. RESULTS AND CONCLUSION:: Compared with the allogeneic bone group, autologous bone group and bone morphogenetic protein composite bone group were better in the maintenance of intervertebral height. Postoperative fusion rates were ranked as follows: composite bone group > autologous bone group > allogeneic bone group (P < 0.05). At 9 and 12 months postoperatively, the fusion rates in the composite bone group were better than those in the autologous bone group (P < 0.05). Nakai scores in the autologous bone and composite bone groups were superior to that in the allogeneic bone group (P < 0.05). There was a significant difference in Nakai scores among the three groups (P < 0.05). These findings indicate that bone morphogenetic protein composite bone is similar to autologous bone but superior to allogeneic bone in the maintenance of intervertebral height, fusion rate and clinical efficacy.