Retrospective analysis of 78 cases with bone defects after bone tumor resection from the Department of Orthopaedics, Guangzhou General Hospital of Guangzhou Military Command from August 1999 to January 2007 was performed. The patients consisted of 47 male and 31 female, with an average age of 21.5 years (7 to 56 years). Among them, 34 cases suffered from giant cell tumor of bone, 12 cases from osteosarcoma, 11 cases from aneurysmal bone cyst, 2 cases from leiomyosarcoma, 1 case from alveolar soft-tissue tumor, and 18 cases from bone fibrous dysplasia. Proximal and distal femur, femoral shaft, proximal tibia, and pelvic were the main pathogenic sites. Bone tumor resections were performed according to Ennekingʼs surgical staging system. Fresh frozen-allografts for reconstruction included massive bone (63 cases), long bone (10 cases), iliac bone (2 cases), and allograft prosthesis composite (3 cases). All patients obtained the follow-up with an average of 37.9 months. The results were assessed by the principle of the criteria proposed by Mankin: excellent in 36 cases, good in 29 cases, fair in 4 cases, and poor in 9 cases. There were 18 cases of local rejections, 4 cases of infection, 5 cases of nonunion, 7 cases of recurrence, and 2 cases of joint instability. The formation of bony callus was observed obviously at the graft union between massive allogeneic bone and allogenic bone at 1 week after transplantation. Graft union and allogeneic bone covered by fine allogeneic and allogenic bones could enhance bone induction. The results suggested that reconstruction of large bone defects with massive bone after tumor resection can achieve satisfactory effect.