OBJECTIVE: To determine the changes in percentage of body fat, cardiovascular fitness, and insulin levels during the 3-month summer break in overweight children enrolled in a school-based fitness program. STUDY DESIGN: Overweight middle-school children were randomized to a lifestyle-focused physical education class (treatment) or standard physical education class (control) for 1 school year (9 months; previously reported). This analysis reports changes during the 3-month summer break in children who participated in the fitness intervention group and who remained at this school the following year and repeated a fitness class. At the beginning and end of the school year, children underwent evaluation of (1) fasting levels of insulin and glucose, (2) body composition by means of dual x-ray absorptiometry, and (3) maximum oxygen consumption as determined by treadmill use. SETTING: Rural middle school and an academic childrenʼs hospital. PARTICIPANTS: Overweight middle-school children. INTERVENTION: School-based fitness curriculum, followed by summer break, and an additional year of school-based fitness intervention. MAIN OUTCOME MEASURES: Cardiovascular fitness test results (maximum oxygen consumption), body composition, and fasting insulin levels. RESULTS: Improvements seen during the 9-month school-year intervention in cardiovascular fitness, fasting insulin levels, and body composition were lost during the 3-month summer break. During this summer break, mean ± SD fitness level decreased (maximum oxygen consumption, −3.2 ± 1.9 mL/kg per minute; P = .007), fasting insulin level increased (+44 ± 69 pmol/L [+6.1 ± 9.7 mIU/mL]; P = .056), and percentage of body fat increased (+1.3% ± 1.3%; P = .02) to levels that were similar to those seen before the school intervention. CONCLUSION: In obese middle-school children, school-based fitness interventions are an important vehicle for health promotion, but without sustained intervention, these benefits may be lost during the extended summer break.