The purpose of this study was to examine the hair loss inhibitory efficacies of ethanolic extracts of Ribes fasciculatum (RFEE) and Callicarpa japonica (CJEE) in 20 mJ/cm2 UVB-irradiated human dermal papilla cells (hDPCs) by measuring transforming growth factor (TGF)-β, interleukin (IL)-1α mRNA, and reactive oxygen species (ROS). In the results of MTT assay, the cell viabllity of hDPCs irradiated with 20 mJ/cm2 UVB was 91.7%, and the results for those for 25 μg/mL treatments with RFEE and CJEE as well as 1 μM MXD after UVB irradiation were over 90%. Thus, the maximum permissible level of both RFEE and CJEE to hDPCs was over 25 μg/mL. In the results of the quantitative RT-PCR assay, treatment with 25 μg/mL RFEE and CJEE, and 1 μM MXD resulted in decreased expression of TGF-β mRNA by 27.3% (p < 0.001), 52.3% (p < 0.001), and 33.9% (p < 0.001), respectively, decreased expression of IL-1α mRNA by 48.7% (p < 0.001), 59.8% (p < 0.001), and 29.1% (p < 0.001), respectively, and decreased production of ROS by 9.5% (p < 0.01), 20.2% (p < 0.001), and 46.8% (p < 0.001), respectively, compared to the control.
The purpose of this study was to examine the hair loss inhibitory efficacies of ethanolic extracts of Ribes fasciculatum (RFEE) and Callicarpa japonica (CJEE) in 20 mJ/cm2 UVB-irradiated human dermal papilla cells (hDPCs) by measuring transforming growth factor (TGF)-β, interleukin (IL)-1α mRNA, and reactive oxygen species (ROS). In the results of MTT assay, the cell viabllity of hDPCs irradiated with 20 mJ/cm2 UVB was 91.7%, and the results for those for 25 μg/mL treatments with RFEE and CJEE as well as 1 μM MXD after UVB irradiation were over 90%. Thus, the maximum permissible level of both RFEE and CJEE to hDPCs was over 25 μg/mL. In the results of the quantitative RT-PCR assay, treatment with 25 μg/mL RFEE and CJEE, and 1 μM MXD resulted in decreased expression of TGF-β mRNA by 27.3% (p < 0.001), 52.3% (p < 0.001), and 33.9% (p < 0.001), respectively, decreased expression of IL-1α mRNA by 48.7% (p < 0.001), 59.8% (p < 0.001), and 29.1% (p < 0.001), respectively, and decreased production of ROS by 9.5% (p < 0.01), 20.2% (p < 0.001), and 46.8% (p < 0.001), respectively, compared to the control.