The purpose of this study was to help singers, directors, and producers create more diverse and grooved performances by analyzing the affecting factor of rhythm groove in singing. For the purpose, among the factors that may affect the groove, such as note length, accent, tempo, pitch, overall sound volume, diction, harmony, and timing, a total of seven elements were selected, including vocal range change, pitch change, BPM (speed) change, note length change, accent change, diction change, and overall volume change, which are elements applicable to singing and Subjects were asked to listen to a baseline sample with no changes in these factors and a sound source sample with changes in these factors, respectively. Then, a survey was conducted asking subjects about the factors that most influenced changes in rhythm groove. The subjects were 110 experts, including music majors, and experiments were conducted by major, such as vocals, guitar, bass guitar, keyboard, drums, and producing (composition, midi, director). It was found that the top four significant factors that most affect changes in rhythm groove in singing are change in note length (41.8%), change in BPM (25.5%), change in accent (19.1%), and change in diction (8.2%). As a point worth noting, when comparing the percentage of vocal sound length and BPM selection in the top three majors of vocals, instrumental music, and composition, the percentages in vocals were 29.9% and 19%, respectively, which are significantly higher than 13% and 6% in instrumental music and composition.