Aims: Although increment application of single-incision laparoscopic cholecystectomy (SILC), it had technical difficulties of physical disturbance and unstable surgical view through the small incision, therefore, we introduced solo single-incision LC (S-SILC) using laparoscopic scope holder, a simple, fixed and easily handled by a surgeon. Methods: A comparison of S-SILC (group A, n=566) and conventional three-incision laparoscopic cholecystectomy (C-TILC, group B, n=874) from January, 2013 to December, 2016 at multicenter was performed. Baseline characteristics, perioperative outcomes including complications were compared. Results: Mean operative time was not significantly different between two groups (P = 0.176), however, S-SILC had more intraoperative GB perforation, especially in initial period (17.0% vs. 2.3%, P < 0.001) and increased usage of additional port (3.2% vs. 0.5%, P < 0.001) and shorter hospital stay (3.3 ± 1.7 vs. 1.9 ± 2.7, P<0.001) than C-TILC. There was no significant difference of major postoperative complications between two groups (P = 0.909) and its identified risk factors were not operation type (P = 0.971), but mean age (P = 0.004) and upper abdominal operation history (P = 0.048). Conclusions: S-SILC is feasible and safe, but careful selection of surgical candidates is necessary, in initial period of S-SILC experience.